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Table 2 Significant effects (after correction for multiple comparison) for all 26 dependent variables in C57BL/6 (C), BALB/c (B), and DBA/2(D) females. All trend effects of Cage Type, i.e. whether housed in single- or mixed-strain trios, are shown in italics even though not significant after the correction. For interactions, effect directions are based on Tukey’s tests for each dependent*independent variable. Denominator degrees of freedom vary as a result of the REML procedure and some sample loss

From: Mixed-strain housing for female C57BL/6, DBA/2, and BALB/c mice: validating a split-plot design that promotes refinement and reduction

Dependent Variable Independent Variable F-value p-value Direction of Effect
Normal Activity Enrichment F1,110 = 27.5 0.0001 Higher if enriched
Stereotypic Behaviour Enrichment F1,115 = 127.8 0.0001 Lower if enriched
Growth Enrichment F1,143 = 14.8 0.0001 Higher if enriched
Mean Corpuscular Volume Enrichment F1,137 = 20.7 0.0001 EH higher
Normal Activity Strain F2,110 = 10.37 0.0001 C = B > D
Stereotypic Behaviour Strain F2,115 = 9.04 0.0002 D = B > C
Novel Object Latency Strain F2,161 = 84.0 0.0001 C > B > D
Startle Response Strain F2,177 = 18.1 0.0001 B > C > D
Forced Swim Test – Duration of Floating Strain F2,159 = 144.6 0.0001 B > C > D
Forced Swim Test – Latency to Begin Floating Strain F2,164 = 86.0 0.0001 D > C = B
Faecal Corticosterone Metabolites Strain F2,99 = 57.6 0.0001 B > D > C
Spleen Weight Strain F2,156 = 35.3 0.0001 B > C = D
Growth Strain F2,143 = 21.5 0.0003 C > D > B
Mean Corpuscular Haemoglobin/Erythrocyte Strain F2,146 = 41.4 0.0001 B > C > D
Mean Corpuscular Haemoglobin Concentration Strain F2,150 = 20.7 0.0001 B > D = C
Mean Corpuscular Volume Strain F2,137 = 109.6 0.0001 C > B > D
Mean Platelet Volume Strain F2,116 = 20.4 0.0001 B = D > C
Absolute Neutrophil Count Strain F2,163 = 11.8 0.0001 C > B = D
Platelet Count Strain F2,123 = 11.1 0.0001 C > D = B
Red Blood Cell Distribution Width Strain F2,120 = 237.1 0.0001 D > B > C
Inactivity Strain*Enrichment F2,105 = 13.6 0.0001 Only D more inactive in EE
Growth Cage Type F 1,175   = 10.8 0.0012 Mixed higher
Forced Swim Test – Duration of Floating Cage Type F 1,159   = 4.36 0.039 Mixed higher
Forced Swim Test – Latency to Begin Floating Cage Type*Enrichment F 1,164   = 4.91 0.028 Post hoc tests found no significant differences between any combination
Absolute Neutrophil Count Cage Type*Enrichment F 1,163   = 4.02 0.047 As above
Faecal Corticosterone Metabolites Cage Type*Strain F 2,99   = 3.17 0.047 Post hoc tests found no difference within strains. Between strain differences are reduced in B and D in mixed-strain cages
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BMC Medical Research Methodology

ISSN: 1471-2288

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