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Table 3 Estimated Cage Type standardized effect sizes (Cohen’s d) that would be required to detect a significant effect with 80 % power (β) and a significance threshold of p = 0.0003 (α). Effect size calculations are based on the least squared means from the original models (i.e. they are based on transformed values that have been corrected for other factors in the model)

From: Mixed-strain housing for female C57BL/6, DBA/2, and BALB/c mice: validating a split-plot design that promotes refinement and reduction

Dependent Variable Empirical Effect Size (d) Estimated Required Effect Size (d)
Normal Activity 0.09 1.24
Inactivity 0.12 1.24
Stereotypic Behaviour 0.27 1.22
Novel Object Latency 0.14 1.04
Startle Response 0.04 0.88
Forced Swim Test – Latency to Begin Floating 0.08 1.10
Forced Swim Test- Duration of Floating 0.32 1.12
Faecal Corticosterone Metabolites 0.20 0.97
Blood Glucose 0.14 0.86
Growth 0.54 2.41
Spleen Weight 0.05 1.12
White Blood Cell Count 0.29 1.21
Red Blood Cell Count 0.23 1.17
Haemoglobin 0.25 1.27
Haematocrit 0.19 1.19
Mean Corpuscular Volume 0.04 1.00
Mean Corpuscular Haemoglobin 0.05 0.93
Mean Corpuscular Haemoglobin Concentration 0.02 0.82
Red Blood Cell Distribution Width 0.10 1.18
Platelet Count 0.11 1.20
Mean Platelet Volume 0.03 1.38
Absolute Neutrophil Count 0.15 0.99
Absolute Lymphocyte Count 0.26 1.17
Absolute Monocyte Count 0.01 1.56
Absolute Eosinophil Count 0.08 1.15
Absolute Basophil Count 0.03 1.38